Are these actually the same, similar or different？
Uridine, Pseudouridine, N1-methylpseudouridine are not the same.
Psuedouridine- psuedouridine synthase rotates the uracil section 180 degrees. Moved 3 carbons over and rotated 180 degrees. Pseudouridine is not N1-Methylpseudouridine. A methyl has been added to N1 position. Pseudouridine is found and can be used as a biomarker in prostate cancer (Uddin 2020)(Uddin, M. B., Wang, Z., & Yang, C. (2020). Dysregulations of Functional RNA Modifications in Cancer, Cancer Stemness and Cancer Therapeutics. Theranostics, 10(7), 3164–3189. https://doi.org/10.7150/thno.41687).
What effect does N1-methylpseudouridine feasibly have?
“ Replacing uridine with m1ψ demonstrates the steric incompatibility of the modified nucleobase with TLR7 binding and immune activation.”(Nance & Meir 2021) (Playing Russian Roulette with Every COVID-19 Injection: The Deadly Global Game. (2022). International Journal of Vaccine Theory, Practice, and Research , 2(2), 619-650. https://doi.org/10.56098/ijvtpr.v2i2.36)
What allosteric effects can it have?
A) This change upstream can alter downstream activity. How is this switch in nucleotides affecting the proposed protein in the injection? Dr. Crick recognized that “folding is simply a function of the order of the amino acids”, known as sequence hypothesis. (Cobb M (2017) 60 years ago, Francis Crick changed the logic of biology. PLoS Biol 15(9): e2003243. https://doi.org/10.1371/journal.pbio.2003243)
Changes to secondary structure in the vaccine derived mRNAs compared to the native virus created with RNAfold. (Source:https://www.researchgate.net/publication/356545068Differences_in_Vaccine_and_SARS-CoV-2 Replication_Derived_mRNA_Implications for_Cell_Biology_and_Future_Disease.)
We can view the difference in the above image. Why? The spike-protein is the common denominator between the coronavirus and the COVID-19 injectable products nevertheless that is the just the introduction. It is not just two modified codons that result “in a version of the spike-protein that is stabilized in its prefusion state (Heinz et al., 2021).” Are the spike proteins produced comparable? Dr Gutschi during her presentation stated that the Wuhan spike had a 141kDa but the spike protein produced by the modfied RNA was 180 kDa. (“Independent Analysis of Quality Issues of the BNT162b2 Biontech/Pfizer vaccine Identified by the European Medicines Agency” by LM Gutschi, BScPhm, PharmD, Oct 26, 2022) What substitutions are being accepted as the same?
A) Is it the difference in the sequences? The following sequences are protein (amino cid) sequences.
Look for the mRNA-1273 sequence and compare?
B) Is it the difference between amino acid compositions? (Daniel Santiago, Solari presentation Nov 2023 and Daniels substack)
C)Is it synonymous substitutions in addition to the replacement of uridine with N1-methylpseudouridine?
There are synonymous changes throughout the BNT162b2 sequence versus Sars CoV-2. Can we call these changes minor or major? Minor changes may have huge results. （Bioavailability oral of ampicillin(30-55%) vs amoxicillin (98.2%), the difference is the -OH (hydroxyl) group is in ampicillin not amoxicillin.)
Ampicillin and amoxicillin are not the same.
D) Is it the difference in the number of base pairs?
Look for the mRNA-1273 sequence and compare?
N1-methylpseudouridine is not the same as uridine, psuedouridine or thymine。 Substituting nucleotides changes the “genetic language”。How is this being interpreted by the human body？
Lab Work：
Video of White clot (or white rubbery structure) removal：
Here is a brief summary of the progress and findings concerning the white clots(or stuctures) found in the video by “HSR” group:
HPLC analyses of white clot (or white structure) sample:
List of top 21 proteins found. 500 plus proteins found in the white clot (or white structure) sample. There is a need to replicate this work.
Is homeostasis disrupted？ From Frameshifting? From the LNP? From the production of aberrant proteins?
(Mulroney, T.E., Pöyry, T., Yam-Puc, J.C. et al. N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting. Nature 625, 189–194 (2024). https://doi.org/10.1038/s41586-023-06800-3)
Kjeldahl method -White clot (or white structure) sample
Amino-acid analyses- New type of protein or disruption in percentage of a normal protein (e.g. collagen).
The team has confirmed both the presence of high concentrations of Phosphorus (P), Sulphur (S), Selenium(Se), Tin (Sn) and Sodium (Na)
My hope is that the human body’s repair mechanisms can kick in. Please review the following reference：
Yu, Z., Chen, J., Ford, B. N., Brackley, M. E., & Glickman, B. W. (1999). Human DNA repair systems: an overview. Environmental and molecular mutagenesis, 33(1), 3–20. https://doi.org/10.1002/(sici)1098-2280(1999)33:1<3::aid-em2>3.0.co;2-l